MouseMet Thermal

mousemet thermal hargreaves hotplate tailflick replacementLESS STRESS FOR THE MOUSE or RAT: MouseMet thermal is a replacement for the Hargreaves or hot plate plantar tests. It is a ramped stimulus that can be applied in the same mouse or rat run as the mechanical stimulus (using filaments or EvF), reducing handling and acclimation time and making a significant contribution to the 3Rs.

Click here for the data sheet for both modalities.

REPEATABLE HEAT TRANSFER: The thermal probe is 2mm diameter and mounted on the measurement arm of a MouseMet EVF transducer. Heating is triggered when the probe is applied to the plantar surface with a force of 1g. This provides consistent thermal transfer without premature withdrawal even when allodynia is present.

topcat metrology mousemet thermal probeAUTOMATIC PEAK HOLD: The probe heats at 2.5C/sec and at threshold the tester simply withdraws the probe. The threshold temperature is then displayed on the readout and held until the reset button is pressed for the next test. There is also a thermal cut-out, factory set to 60C but adjustable by the user.

VALIDATION: The system has been trialled by the University of Queensland where it produced baseline thresholds of 50C on mice and detected both heat allodynia (at 43C) and partial analgesia. It builds on Topcat Metrology’s 20 years experience in the field of thermal nociceptive testing on all species from rats to horses. Click here for the validation paper.


mousemet thermal hargreaves tail flick hotplate replacement

INSENSITIVE TO HAND TREMOR: As with MouseMet EvF, the thermal transducer eliminates the effects of operator hand tremor: the probe is flexible in the horizontal plane and can move slightly from side to side without affecting the thermal contact. In the vertical plane, the transducer can absorb small vertical deflections without changing the probe application force significantly.

EASY PLACEMENT & GOOD VISIBILITY: The probe is applied to the plantar surface by rotating the transducer body by the black handles. This is an intrinsically easy action for the operator to perform, with the elbows comfortably supported on the bench top and the mouse run at the correct height.

Mouse runs are supplied in groups of 2, 4 or 6 with a height adjustable stand. Rat runs are supplied individually. The one dimensional design encourages the rodent to sit side on to the operator, which maximises access to the plantar surface and gives the operator an excellent view of the probe tip and the paw.

TWO MODALITIES IN ONE ENVIRONMENT: Mechanical thresholds may also be measured in the same runs using the MouseMet EVF transducer. This eliminates the acclimation time from moving to a new testing environment, thereby reducing the stress on the rodent.

As well as outright purchase, the system is available for 6 month lease, with an option to buy at the end. For prices and availability, please email mike@topcatmetrology.co.uk